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1800-102-2727Have you ever seen how long chains of flowers are made for decorating the house during festivals? Flowers are added one after another and weaved together using a thread to form a long chain. Did you know that the DNA in your cell is assembled in a similar way? It is made up of repeating units of a compound known as nucleotide and is therefore considered as a polynucleotide chain. The bond between the nucleotides is the thread that joins them together.
But not just DNA, even RNA is composed in a similar way but the nucleotides in DNA and RNA are different in many respects. In fact, not all the nucleotides in a DNA or RNA are identical to each other, they vary in their composition. The variable component of a nucleotide is the nucleoside which is nothing but the nucleotide devoid of its phosphate group. In this article we will discuss the nucleotides and their nucleosides and how they differ from each other.
Table of Content:
The monomeric subunits of nucleic acids such as DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) are known as nucleotides. Nucleotides are composed of -
The presence of a phosphate group (PO43-) makes them acidic at physiological pH. Nucleotides can be mone, di or triphosphates having one, two or three phosphate groups attached to the 5’OH group of the pentose sugar.
Fig: Phosphate group
An N-glycosidic linkage helps the nitrogenous base to bind to the 1’ carbon of the pentose sugar. A phosophoester bond helps the phosphate group to attach to the 5’ hydroxyl (-OH) group of the pentose sugar.
Fig: Structure of a nucleotide
A phosphodiester bond connects the 5’ phosphate group of one nucleotide to the 3’ hydroxyl group r of the adjacent nucleotide in a polynucleotide chain.
Fig: Phosphodiester bond
Pentose sugar is a 5 carbon atom containing monosaccharide. Based on the pentose sugar, nucleotides can be of two types - ribonucleotides and deoxyribonucleotides. Ribonucleotides have ribose sugar (having the 2’hydroxyl (-OH) group) whereas deoxyribonucleotides have deoxyribose sugar (oxygen is missing at the 2’ carbon position and only 2’ hydrogen (H) is present). Ribonucleotides are the monomeric unit of RNA (Ribonucleic acid) and deoxyribonucleotides are the monomeric units of DNA (Deoxyribonucleic acid).
Fig: Examples of pentose sugar found in nucleic acids
These are nitrogen containing compounds having heterocyclic ring structures. Based on the heterocyclic rings present, nitrogenous bases can be:
Fig: Different types of nitrogenous bases
Deoxyribonucleotides in DNA can have either adenine, guanine, cytosine or thymine as nitrogenous bases. Ribonucleotides in RNA have either adenine, guanine, cytosine or uracil as nitrogenous bases. In RNA, thymine is replaced by uracil.
Fig: Nitrogenous bases - Purines
Fig: Nitrogenous bases - Pyrimidines
A nucleotide without the phosphate group, that is, a pentose sugar having a nitrogenous base attached to its 1’ carbon is known as a nucleoside. A N-glycosidic bond helps the 1’ carbon of the pentose sugar to bind to the 1st nitrogen of the pyrimidines and 9th nitrogen of the purines.
Fig: Nucleoside
Addition of a phosphate group to the 5’OH group of the pentose sugar in a nucleoside, converts it into a nucleotide.
Sugar + Nitrogenous base |
Name of the nucleoside |
Ribose + Adenine |
Adenosine |
Ribose + Guanine |
Guanosine |
Ribose + Cytosine |
Cytidine |
Ribose + Uracil |
Uridine |
Sugar + Nitrogenous base |
Name of the nucleoside |
Deoxyribose + Adenine |
Deoxyadenosine |
Deoxyribose + Guanine |
Deoxyguanosine |
Deoxyribose + Cytosine |
Deoxycytidine |
Deoxyribose + Thymine |
Deoxythymidine |
Nucleoside + Phosphate |
Name of the nucleotide |
Adenosine + Phosphate |
Adenylic acid or Adenosine monophosphate (AMP) |
Guanosine + Phosphate |
Guanylic acid or Guanosine monophosphate (GMP) |
Cytidine + Phosphate |
Cytidylic acid or Cytidine monophosphate (CMP) |
Uridine + Phosphate |
Uridylic acid or Uridine monophosphate (UTP) |
Nucleoside + Phosphate |
Name of the nucleotide |
Deoxyadenosine + Phosphate |
Deoxyadenylic acid or Deoxyadenosine monophosphate (dAMP) |
Deoxyguanosine + Phosphate |
Deoxyguanylic acid or Deoxyguanosine monophosphate (dGMP) |
Deoxycytidine + Phosphate |
Deoxycytidylic acid or Deoxycytidine monophosphate (dCMP) |
Deoxythymidine + Phosphate |
Deoxythymidylic acid or Deoxythymidine monophosphate (dTMP) |
Nucleotide |
Nucleoside |
Nucleotides are composed of a phosphate group, a sugar and a nitrogenous base. |
Nucleosides are composed of a sugar and a base without the phosphate group. |
They are one of the major causes of cancer-causing agents to this very day. |
They are used as agents in medicine that are primarily used against viruses and cancer-causing agents. |
Examples of ribonucleotides are
|
Examples of ribonucleosides are adenosine, guanosine, cytidine and uridine. |
Examples of deoxyribonucleotides are -
|
Examples of deoxyribonucleosides are deoxyadenosine, deoxyguanosine, deoxycytidine and deoxythymidine. |
1. Cytidylic acid is composed of
Solution: Cytidylic acid is a ribonucleotide formed by association between Cytidine and phosphate. Cytidine is the nucleoside made up of ribose sugar and cytosine and it combines with a phosphate group at its 5’OH group to form cytidylic acid. Thus the correct option is c.
2. Which of the following is not found in an RNA molecule?
Solution: RNA is composed of repeating units of ribonucleotides which are made up of a ribose sugar, nitrogenous base and a phosphate group. The nitrogenous bases found in RNA are adenine, guanine, cytosine and uracil (in place of thymine). Thus, the ribonucleotides found in RNA are -
Deoxythymidylic acid is a deoxyribonucleotide found in DNA. Thus, the correct option is b.
3. Which of the following has a double ring structure?
Answer: Purines have a double ring structure consisting of a hexagonal pyrimidine ring and an pentagonal imidazole ring, examples are adenine and guanine. Cytosine, uracil and thymine are pyrimidines with a single hexagonal ring. Thus, the correct option is a.
4. Which of the following options is correct?
Solution: The monomeric subunits of nucleic acids such as DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) are known as nucleotides. Nucleotides are composed of -
An N-glycosidic linkage helps the nitrogenous base to bind to the 1’ carbon of the pentose sugar. A phosophoester bond helps the phosphate group to attach to the 5’ hydroxyl (-OH) group of the pentose sugar.
Fig: Structure of a nucleotide
A nucleotide without the phosphate group, that is, a pentose sugar having a nitrogenous base attached to its 1’ carbon is known as a nucleoside. Thus, a nucleoside and a phosphate group together form a nucleotide and the correct option is c.
Answer: cAMP or cyclic AMP is adenosine monophosphate that functions as a second messenger in the transmission of neural or hormonal messages to the cell. When certain protein or polypeptide hormones bind to the surface receptor of plasma membrane, they stimulate generation of cAMP from ATP by the action of adenylate cyclase enzyme. cAMP activates protein kinase enzymes which add phosphate groups to intracellular enzymes, thus resulting in their activation or deactivation.
Answer: Nucleotides are mon, di- or triphosphates of nucleosides. When there are more than one phosphate groups attached to the nucleoside, they are known as higher nucleotides. For example, ADP and ATP. Linkage of additional phosphate groups involves a large amount of energy.
Answer: A coenzyme is an organic carrier molecule which cooperates with a particular enzyme in catalysing a biochemical reaction. Coenzymes are formed from the nucleotides by replacing the nitrogenous base with a vitamin. For example, NAD (Nicotinamide adenine dinucleotide).
Answer: DNA is a double stranded helical molecule and the two strands of the double helix are held together by hydrogen bonds between the nitrogenous bases of the nucleotides of opposite strands. Adenine pairs with thymine using two hydrogen bonds and guanine pairs with cytosine using three hydrogen bonds.
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