By Team Aakash Byju's | 31st January 2023

Separation of DNA Fragments on Electrophoresis

?

The commonly used matrix is agarose gel.

The DNA fragments move towards the anode under an electric field through the matrix.

The DNA fragments resolve according to their size.

None of the above.

Which one of the following statements is wrong with respect to the separation of DNA fragments on electrophoresis?

A.

B.

C.

D.

Detailed Explanation

The correct answer is  D) None of the above.

Arrow

The DNA molecule on the agarose gel matrix is mainly separated by agarose gel electrophoresis.

The deeply carved wells on the gel are loaded with negatively charged DNA molecules.

A strong electric field that is applied to the loaded sample causes the DNA to separate based on its mass and charge.

The DNA molecules arrange according to mass since they all have the same charge.

The smaller DNA molecules move faster and get aligned at the top, with the larger segment at the bottom.