By Team Aakash Byju's
It happens when the cell is in a condition of competence, which implies it can take in external material.
The first step starts with preparing competent cells that need to be transformed.
Once the cells are competent, they are subjected to another cycle of heat shock in the presence of plasmid DNA.
The transformed cells are then cultivated again to boost cell viability and molecular cloning efficiency.
Finally, transformed cells are plated with the resources necessary for identifying and recovering the desired transformants.